Hu et al., Anal. Chem. 2023 May 16;95(19) 7665-7675 used LiquidScan to affinity capture EVs from plasma of breast cancer patients.
Extracellular vesicles (EVs) carry RNA cargo that is believed to be associated with the cell-of-origin and thus have the potential to serve as a minimally invasive liquid biopsy marker for supplying molecular information to guide treatment decisions (i.e., precision medicine). LiquidScan was used to affinity isolate EV subpopulations with monoclonal antibodies attached to the surface of a microfluidic chip. The EV microfluidic affinity purification (EV-MAP) chip was used for the isolation of EVs sourced from two-orthogonal cell types and was demonstrated for its utility in a proof-of-concept application to provide molecular subtyping information for breast cancer patients.
